human skin fibroblasts hsf cell line  (ATCC)

Journal: Bioengineering & Translational Medicine

Article Title: Fibroblasts and endothelial cells interplay drives hypertrophic scar formation: Insights from in vitro and in vivo models

doi: 10.1002/btm2.10630

Figure Lengend Snippet: The interaction between fibroblasts and endothelial cells in indirect coculture system. (a) CCK8 assay was performed to detect the viability of fibroblast ( n = 6). (b) Transwell ( n = 12) was performed to detect the migration of fibroblast. (c) Scratch assays ( n = 15) were performed to detect the migration of fibroblast. (d) ELISA results show that the levels of VEGF, TGFβ, PDGF, and bFGF in EC‐CM. (e) Scratch assays ( n = 15) and Transwell ( n ≥ 6) were performed to detect the migration of endothelial cells. (f) ELISA results show that the levels of VEGF, TGFβ, PDGF, and bFGF in SCF‐CM and HSF‐CM (* p < 0.1, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared with control group; scale bar: 200 μm).

Article Snippet: Human skin fibroblast cell line (HSF, iCell) was cultured under 37°C in 5% CO 2 conditions in complete medium (DMEM + 10% FBS + 2% P/S).

Techniques: CCK-8 Assay, Migration, Enzyme-linked Immunosorbent Assay, Control

Journal: Bioengineering & Translational Medicine

Article Title: Fibroblasts and endothelial cells interplay drives hypertrophic scar formation: Insights from in vitro and in vivo models

doi: 10.1002/btm2.10630

Figure Lengend Snippet: Differential expression genes analysis of fibroblast in co‐culture multicellular spheroids compared to monoculture cell spheroids. (a) Volcano plot of DEGs. (b) GO analysis showed these differentially expressed genes were enriched in extracellular matrix (green font), angiogenesis (red font), cell behavior‐related (blue font), and inflammatory‐related (yellow font) related GO terms. (c) Enrichment plots of KEGG pathway for representative signaling pathways using the DEGs.

Article Snippet: Human skin fibroblast cell line (HSF, iCell) was cultured under 37°C in 5% CO 2 conditions in complete medium (DMEM + 10% FBS + 2% P/S).

Techniques: Quantitative Proteomics, Co-Culture Assay, Protein-Protein interactions

Journal: Bioengineering & Translational Medicine

Article Title: Fibroblasts and endothelial cells interplay drives hypertrophic scar formation: Insights from in vitro and in vivo models

doi: 10.1002/btm2.10630

Figure Lengend Snippet: Cell–cell communications between fibroblasts and endothelial cells in scars and relatively normal skin tissues. (a) Workflow. Samples were harvested from nine individuals for scRNA‐seq. (b) UMAP plot of 50,941 human cells, colored by cluster. (c)–(e) Bubble Plot, Violin plot, and UMAP demonstrate the expression level and distribution of specific genes in different cell types. (f) Heatmap depicting interactions among all cell types obtained with CellPhoneDB. (g) Dot plot showing the representative ligand–receptor pairs between fibroblast and endothelial cells in normal skin versus scar. The dot size represents the p value. The dot color represents the mean of the average expression level of the two interacting molecules in their respective clusters. (h) Scatter plot displaying the upregulated receptor and ligand genes by GO enrichment analysis. The rich factor indicates the ratio of the number of genes with significant differences annotated as a specific GO term to all genes annotated as the same GO term. Dot size represents the number of genes with a significant difference enriched in a specific GO term. The dot color represents the p value obtained by GO analysis. p < 0.05 was used to indicate significant enrichment.

Article Snippet: Human skin fibroblast cell line (HSF, iCell) was cultured under 37°C in 5% CO 2 conditions in complete medium (DMEM + 10% FBS + 2% P/S).

Techniques: Expressing